Association of PPARGC1A and CAPNS1 gene polymorphisms and expression with meat quality traits in pigs

Gandolfi G., Cinar M. U., Ponsuksili S., Wimmers K., Tesfaye D., Looft C., ...Daha Fazla

MEAT SCIENCE, cilt.89, sa.4, ss.478-485, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 89 Sayı: 4
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1016/j.meatsci.2011.05.015
  • Dergi Adı: MEAT SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.478-485
  • Anahtar Kelimeler: PPARGC1A, CAPNS1, Meat quality, DNA polymorphisms, Gene expression, Pigs, WATER-HOLDING CAPACITY, DRIP LOSS, TRANSCRIPTIONAL COACTIVATOR, MOLECULAR CHARACTERIZATION, LONGISSIMUS-DORSI, CALPAIN ACTIVITY, BOVINE PPARGC1A, GENOME SCAN, MUSCLE, PORK
  • Erciyes Üniversitesi Adresli: Hayır

Özet

This study aimed to investigate the genes PPARGC1A (peroxisome proliferator-activated receptor gamma-coactivator 1A) and CAPNS1 (calpain small subunit 1) as candidate genes affecting meat quality traits in pigs. Four polymorphisms were identified in PPARCG1A and three in CAPNS1. The PPARGC1A polymorphism c.1288T>A was associated with pH and cooking loss in a F2 Durocx Pietrain experimental cross (DuPi, n =313) and with pH values in Italian Large White (ILW, n=380) and Italian Landrace (ILA, n=158) populations (P<0.05). The CAPNS1 polymorphism c.429A>C was associated with pH and conductivity in DuPi and with meat color in ILA (P<0.05). PPARGC1A mRNA expression associated with drip loss (P<0.01) and the same tendency was found for CAPNS1 (P= 0.06). The promoter methylation profiling suggested that methylation is not involved in CAPNS1 expression regulation. In conclusion, porcine PPARGC1A and CAPNS1 genes may affect meat quality traits, with breed-specific differences, and they could be used as markers for the improvement of meat quality in pigs. (C) 2011 Elsevier Ltd. All rights reserved.