© 2020 Refik Saydam National Public Health Agency (RSNPHA).Objective: Nanoparticles have been used in many fields including biotechnology, pharmacology, medicine, sensors, informatics and communications, electronics, defense, textile, machinery and construction industries due to their superior physical properties. Biological synthesis methods have been widely used in recent years due to their environmentally friendly properties for pharmaceutical and other biomedical applications since toxic chemicals are not used. Studies on the biological synthesis of microorganisms and plant extracts and nanoparticles have increased rapidly today. Plant origin polyphenolic compounds have been used extensively in recent studies. In this study, it was aimed to investigate the effect of biosynthetic silver nanoparticles prepared with grape seed extract on intestinal damage and apoptosis caused by lipopolysaccharide (LPS). Methods: In the study, 80 rats of Wistar albino were male rats; control group, LPS group, grape seed extract group, silver nanoparticle (AgNP) group, Ag ion group and LPS + Ag ion group, LPS + AgNP group, LPS + grape seed extract groups were randomly divided into eight groups. Apoptotic cell counts were evaluated by histopathological evaluation and TUNEL method in intestinal tissues from animals. Results: As a result of the study, it has been observed that LPS application causes lumen pouring in necrotic villi in the intestines, degenerative changes and bleeding in villi, and these changes are alleviated with the application of Ag ion, grape seed extract and AgNPs together with LPS. In addition, LPS significantly increased the number of apoptotic cells compared to other groups, and the application of Ag ions, grape seed extract and AgNPs together with LPS showed a statistically positive effect. Conclusion: As a result, It can be said that biosynthetic AgNPs have the potential to reduce the toxicity caused by LPS. This study will shed light on future studies.