Effects of treating with auxin solutions on rooting of cuttings of sainfoin (Onobrychis viciifolia)

Avcı S., Çöçü S. , Aasım M., Sancak C., Özcan S.

TROPICAL GRASSLANDS, vol.44, pp.123-127, 2010 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 44
  • Publication Date: 2010
  • Title of Journal : TROPICAL GRASSLANDS
  • Page Numbers: pp.123-127


Sainfoin is a cross-pollinating plant and segregates when material is multiplied from seed during breeding programs. While the use of cuttings allows rapid and reliable multiplication of selected material for vegetative propagation, cuttings often do not root well. This study examined the root development of sainfoin cuttings after dipping in solutions containing 0, 25, 50, 100 and 200 mg/L indole-3-acetic acid (IAA), alpha-naphthalene acetic acid (NAA) and indole-3-butyric acid (IBA) for 8, 16 and 24 h. The hormones were dissolved in either liquid Murashige and Skoog (MS) medium or distilled water. While untreated cuttings produced virtually no roots, all auxin treatments stimulated rooting on some cuttings. Dipping treatments using liquid MS medium as the base for the auxin solution produced better root development than those using distilled water. IBA was less effective than IAA or NAA in stimulating root production on cuttings. Dipping cuttings in a solution of IAA at 100 mg/L for 8 h in liquid MS medium would seem to be adequate to produce satisfactory root development. Rooted plantlets were transferred to pots and grew successfully under greenhouse conditions.