Leishmaniasis is widely seen in tropical and subtropical regions of the world. It is caused by obliged intracellular protozoa of the genus Leishmania and is responsible for three clinical manifestations: cutaneous leishmaniasis (CL), visceral leishmaniasis (VL) and mucocutaneous leishmaniasis (ML). CL is a skin infection and is the most common form of leishmaniasis. Cl is rarely fatal but it may lead to permanent scars. CL is endemic in the western and southeastern parts of Turkey. This study was aim to investigate the presence of Leishmania amastigotes in CL suspected patients who applied to Erciyes University Medical Faculty Department of Parasitology, in Kayseri, central Anatolia of Turkey, between January 1995 and August 2012. Scraping of the ulcer samples obtained from 53 (47.7%) males and 58 (52.3%) females making a total of 111 CL suspected patients were examined by Giemsa stain. Microscopic examination showed Leishmania amastigotes in 43 (38.7%) patients. 36 patients from Kayseri, 4 patients from Nigde, one patient from Nevsehir, one patient from the Netherlands and one patient from Afghanistan a total 43 patients with CL, were admitted to our hospital. 14 out of 36 patients from Kayseri admitted to our hospital resides in Yahyali. As a result, CL is still an important health problem in Kayseri, particularly in the district of Yahyali.