In this study, we have explored the prospect of assessing and following level of total denture adaptation by use of EMG signals recorded during gum chewing. Total of 14 edentulous patients, 6 women and 8 men, with an average age of 63 +/- 9 years, were recruited. Separate EMG recordings were obtained from left and right temporalis and masseter muscles of the patients for a period of 10 seconds, while they were chewing a sugar-free gum on their left and right sides. EMG recordings were repeated at three times: before, right after, and six months after the placement of the denture. We have tried to standardize environmental and individual factors during EMG recordings. The EMG data have been pre-processed and analyzed using Discrete Wavelet Transform (DWT) and obtained features were statistically evaluated using the paired sample t-test. Chewing activity on the right and left side is analyzed by making comparisons of muscle activity between before and right-after cases and before and six-months-after denture fixation cases. A comparison between right and left side mastication is also made at different time points. We have suggested and implemented a new test and comparison procedure in order to assess adaptation to denture fixation using EMG analysis. In this study, the results indicate that DWT based EMG analysis is instrumental in evaluating denture adaptation and as time progresses the adaptation to denture and hence chewing efficiency increases in patients with total denture replacement.