Raman spectroscopy: A novel experimental approach to evaluating cisplatin induced tissue damage


Creative Commons License

Yay A. H., Onses M. S., Sahmetlioglu E., Ceyhan A., Pekdemir S., Onder G. Ö., ...More

TALANTA, vol.207, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 207
  • Publication Date: 2020
  • Doi Number: 10.1016/j.talanta.2019.120343
  • Journal Name: TALANTA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, L'Année philologique, Aerospace Database, Analytical Abstracts, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, Linguistic Bibliography, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Keywords: Cisplatin, Ovary, Curcumin, Beta-carotene, Raman spectroscopy, ACUTE RENAL INJURY, INDUCED NEPHROTOXICITY, OXIDATIVE STRESS, MOLECULAR-MECHANISMS, CANCER-THERAPY, CURCUMIN, INFLAMMATION, SPECTRA, KIDNEY, LIVER
  • Erciyes University Affiliated: Yes

Abstract

The aim of this work is to clarify the effect of curcumin and beta-carotene on cisplatin-induced tissue damage and to demonstrate the potential of Raman spectroscopy to detect tissue changes consistent with liver and kidney histopathology as a potential diagnostic adjunct. In the study, 56 Wistar albino female rats were used and randomly divided into 7 groups (n:8). Sham group received only sesame oil; Cisplatin group, received a single dose injection of cisplatin; Beta-carotene group, treated with beta-carotene orally; Cisplatin + Beta-carotene group, pretreated with beta-carotene 30 min prior to the cisplatin injection, then received cisplatin; Curcumin group, orally treated with curcumin; Cisplatin + Curcumin group, pretreated with curcumin 30min prior to the cisplatin injection, then received cisplatin. The second application was performed 1 week after the first application. One of the liver and kidney tissues was taken to 10% form for histopathological examinations and the others were taken to -80 degrees C for raman spectroscopy. Received sections were hematoxylin-eosin stained. The avidin-biotin peroxidase method was used for to investigate anti-TNF-alpha and IL1-beta activities. TUNEL method was applied to determine apoptotic cells.