Genome analysis of a new potential probiotic strain Lactiplantibacillus plantarum DY46 isolated from fermented turnip


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Ortakcı F., Yetiman A. E. , Kotil S. E.

International Biotechnology Congress (BIO 2021), İstanbul, Turkey, 9 September - 11 October 2021, pp.8

  • Publication Type: Conference Paper / Summary Text
  • City: İstanbul
  • Country: Turkey
  • Page Numbers: pp.8

Abstract

INTRODUCTION: Lactiplantibacillus plantarum is widely used as probiotic culture in

dairy food applications such as milk, yogurt, yogurt drink etc. A new Lb. plantarum

strain DY46 was isolated from a traditionally fermented liquid product called shalgam

from the Southern region of Anatolia following incubation on MRS agar at 30°C for 5

days. RESULTS: DY46 is gram positive, short rod and catalase negative. This

bacterium fermented 22 of the 50 substrates tested on API CH50 fermentation panels.

To learn more about the metabolic capabilities of DY46, whole genome sequencing

was performed using Illumina Miseq platform. The sequences were assembled into a

3.32 Mb draft genome using PATRIC 3.6.8. (https://patricbrc.org/app/Assembly2)

consisting of 153 contigs, and preliminary genome annotation was performed using

the RAST algorithm (rast.nmpdr.org). The DY46 genome consists of a single circular

chromosome of 3,332,827 bp that is predicted to carry 3219 genes, including 61 tRNA

genes, 2 rRNA operons. The genome has a GC content of 44.3% includes 98 predicted

pseudogenes, 25 complete or partial transposases and 3 intact prophages. DY46

genome also predicted to carry genes of PlantaricinE, PlantaricinF and PlantaricinK

showing antimicrobial potential of this bacterium which can be linked to in vitro

antagonism tests that DY46 can inhibit dairy food pathogens of Bacillus cereus ATCC

33019, Escherichia coli ATCC 25922. Acid and bile tolerance of DY46 revealed this

strain could potentially pass thru the stomach and reach into gut for providing probiotic

therapeutic affects to health.

Keywords: Probiotic, fermented turnip, genome, antimicrobial