Akademik Veri Yönetim Sistemi
10th International Academic Studies Conference, Manisa, Türkiye, 28 - 30 Kasım 2022, ss.176
Burn injuries cause impairment of skin integrity resulting in bacterial
infections. Various antibiotic treatments are applied for infections, however
antibiotic effectiveness decreases gradually owing to antibiotic resistance. In
our study, firstly the inhibitory effect of homogeneous mixture including
lactic acid bacteria; Lactobacillus plantarum, Lactobacillus acidophilus,
Lactobacillus rhamnosus on Pseudomonas aeruginosa, Escherichia coli,
Staphylococcus aureus pathogens was investigated. To load probiotic
bacteria and provide suitable applying, thermosensitive in situ gel formulation
was prepared and its characterization tests were conducted. Gelation time and
temperature of optimum formulation were 49±43 and 32.33±0.66, respectively and
viscosity at room temperature and body temperature were 134.00±3.21 cP and
840.26±67 cP, respectively. Stabilization results at 5±3°C for 20 days showed
that our probiotic bacteria could effectively grow in Pluronic F 127 in situ
gel formulation. The local
administration of Pluronic F-127 in-situ forming gel containing Lactobacillus
species mixture is suggested to be a promising approach for the treatment of
burn wounds.