The effects of boron and plant growth promoting bacteria (Bio-B) on the some of physiological parameters with amino acid and organic acid of strawberry cv. Fern were investigated under field conditions. The study treatments were soil, foliar, and soil+foliar applications as main plot and two Bio-B (control and plant growth promoting rhizobacteria; Azospirillum brasilense 1x10(9) cfu ml(-1)+10% boron as Di-SodyumOktaboratTetraHidrat) fertilizer applications as subplot in complete randomized block design with four replicates. Bio-B fertilizer was applied in three methods such as soil, foliar and soil+foliar application methods on strawberry. Two years data from the present study reported that the use of Bio-B significantly increased chlorophyll, photosynthesis, stomatal and membrane permeability, amino acid and organic acid of strawberry leaf. Soil+foliar applications of Bio-B fertilizer increased to strawberry yield compared to the control by 65.9%. Foliar applications of Bio-B fertilizer increased chlorophyll and stomatal permeability in comparison to the control by 17.62 and 56.52%, respectively. However, soil+foliar application of Bio-B fertilizer increased membrane permeability and photosynthesis compare to those under the control 24.80 and 23.32%, respectively. Amino acid content of strawberry was increased (23.45%) and organic acid amount was increased (23.45%) under soil application of Bio-B fertilizer compare to those under the control. The results of this study concluded that Bio-B fertilizer application was positively associated with strawberry yield and increased the potential to chlorophyll, photosynthesis, stomatal and membrane permeability, amino acid, organic acid content under field conditions.