© 2021, Centenary University. All rights reserved.Citrus is one of the most important fruit species that is cultivated and has a high species diversity. Consuming fresh and using as a raw material in different industries increases the importance of citrus fruits. Therefore, development of new varieties and rootstocks in citrus fruits is very important. Plant tissue culture studies are a powerful alternative to reproduce valuable plants obtained as a result of crossbreeding studies. In this study, the effect of genotype and media on micropropagation performances of Sunki mandarin X Rubidoux trifoliate hybrids (Murashige and Skoog Medium -MS, Woody Plant Medium -WPM, Rugini Olive Medium-ROM) were investigated. In addition, the effect of genotype and different plant growth regulators (indole butyric acid - IBA and naphthalene acetic acid - NAA) on rooting of plants was investigated. Rooted plants acclimatized to greenhouse conditions. Among the genotypes, the most successful genotypes were SR-60 and SR-75 in the micropropagation stage, while the most successful genotypes were SR-41, SR-47 and SR-60 in the rooting stage. The effect of nutrient media on micropropagation was found statistically insignificant (P <0.05). In rooting assay, the most successful plant growth regulator for plant height and root length was determined as IBA, and the most successful plant growth regulator for root number was determined as NAA.