Nanotechnology in Plant Disease Management: DNA-Directed Silver Nanoparticles on Graphene Oxide as an Antibacterial against Xanthomonas perforans


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Ocsoy I., PARET M. L., Ocsoy M. A., KUNWAR S., Chen T., YOU M., ...More

ACS NANO, vol.7, no.10, pp.8972-8980, 2013 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 7 Issue: 10
  • Publication Date: 2013
  • Doi Number: 10.1021/nn4034794
  • Journal Name: ACS NANO
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.8972-8980
  • Keywords: AgNPs, graphene oxide, Ag@dsDNA@GO composites, dsDNA, bacteria, tomato, CAMPESTRIS PV-VESICATORIA, BACTERIAL SPOT, ESCHERICHIA-COLI, RESISTANCE, TOMATO
  • Erciyes University Affiliated: No

Abstract

Bacterial spot caused by Xanthomonas perforans is a major disease of tomatoes, leading to reduction in production by 10-50%. While copper (Cu)-based bactericides have been used for disease management, most of the X. perforans strains isolated from tomatoes in Florida and other locations worldwide are Cu-resistant. We have developed DNA-directed silver (Ag) nanoparticles (NPs) grown on graphene oxide (GO). These Ag@dsDNA@G0 composites effectively decrease X. perforans cell viability in culture and on plants. At the very low concentration of 16 ppm of Ag@dsDNA@G0, composites show excellent antibacterial capability in culture with significant advantages in improved stability, enhanced antibacterial activity, and stronger adsorption properties. Application of Ag@dsDNA@G0 at 100 ppm on tomato transplants in a greenhouse experiment significantly reduced the severity of bacterial spot disease compared to untreated plants, giving results similar to those of the current grower standard treatment, with no phytotoxicity.