Are Epidermal Growth Factor Receptors and Ligands expressed in the Spleen of the Japanese Quail (Coturnix coturnix japonica) during the Post-hatching Period?

Alan E. , Liman N. , Sağsöz H.

30th European Association of Veterinary Anatomists Congress, Cluj-Napoca, Romanya, 23 - 26 Temmuz 2014, cilt.43, ss.20

  • Yayın Türü: Bildiri / Özet Bildiri
  • Cilt numarası: 43
  • Basıldığı Şehir: Cluj-Napoca
  • Basıldığı Ülke: Romanya
  • Sayfa Sayıları: ss.20


Introduction: The Epidermal Growth Factor (EGF) family

(ErbBl, ErbB2, ErbB3, ErbB4 receptors and their ligands

such as EGF, neuregulin and amphiregulin) plays an

im portant role in the development of several organs

(Ejskjaer K, et al. 2005: Mol. Hum. Reprod. 11, 543-551).

The avian spleen is a principal organ of systemic im m unity

and its importance in disease resistance is presumably

accentuated by the scarcity of avian lymph nodes

(John JL. 1994: Q. Rev. Biol. 69, 327-351). The aim of

this study was to determine whether ErbB receptors and

their ligands are expressed in the structural component of

the quail spleen during the post-hatching period.

Methods: Five quails each, aged 1, 7, 14, 21, 28, and

60 days, were euthanized under ether anaesthesia and their

spleens were fixed in a formaldehyde-alcohol solution. Following

routine histological processing, strepavidin-biotinperoxidase

method was used for immunohistochemical


Results: EGF was not observed in any of the spleens structural

components. Although, strong cytoplasmic immunoreactions

for ErbB2, ErbB4 and Neuregulin were

observed in ellipsoid associated cells of the quail spleen

throughout the post-hatching period, amphiregulin immunostaining

was weak. Furthermore, it was observed

that ErbB2 immunoreactivity in ellipsoid associated cells

increased after the 7th day post-hatching. ErbB1, ErbB3,

ErbB4, amphiregulin and neuregulin immunoreactions

were relatively similar in all components of the spleen

during post-hatching period. Some cells of the peri-arterial

lymphatic sheath (PALS) and peri-ellipsoidal white

pulp (PWP) showed positive immunoreactivity for ErbB2,

ErbB3, ErbB4, Neuregulin and Amphiregulin, but not

ErbB1. ErbB2 immunoreactivity in the muscle cells of

vessels was stronger than ErbB1, ErbB4, neuregulin and

amphiregulin immunoreactivities.

Conclusions: Our data show that EGF is not produced by

the spleen, however, ErbB1, ErbB2, ErbB3, ErbB4, amphiregulin

and neuregulin are expressed by different structural

components of the quail spleen during the posthatching


This study was approved by the Ethics Committee of

Erciyes University.