An Investigation of Transport Properties of Liposomes and Nanoparticles Containing Doxycycline and Soduim Taurocholate Through Caco-2 Cell Line


YÜCEL Ç. , Yılmaz Ş., Değim Z.

16 th IPTS, Türkiye, 1 - 04 Eylül 2012, ss.93

  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.93

Özet

Doxycycline is a semisynthetic antibiotic with a broad
spectrum of activity against a wide range of gram negative
and positive pathogens(1). The present data in the literature
indicate that doxycycline as a cytotoxic agent and matrix
metalloproteinases inhibitor can be a reasonable candidate
for the treatment of glioblastoma multiforme(2).
Liposomes could act as biocompatible, biodegradable,
non-immunogenic drug carriers, the advantages of
liposomal-encapsulated drugs are prolonged duration of
exposure(3). Sodium taurocholate (NaTC) increased paracellular
absorbtion due to opening of tight junctions between
epithelial cells(4). Nanoparticles are submicron-sized polymeric
colloidal particles with a therapeutic agent of interest
encapsulated within their polymeric matrix or adsorbed
or conjugated onto the surface(5). Therefore NaTC was also
used as penetration enhancer in liposome and nanoparticle
formulation.
Caco-2 cells are human colon cancer derived cells, which
are increasingly used in in-vitro blood-brain barrier (BBB)
models in passive permeability and membrane transport
studies(6,7).
In this study, Caco-2 cells were used as a model. The cytotoxic
effects of doxycycline on Caco-2 cells, the transport
of doxycycline nanoparticle and liposome formulations
through Caco-2 cells were investigated. 3-[4,5-dimethylthiazol-
2-yl]-2,5-diphenyltetrazolium bromide (MTT) test was
performed to test doxycycline effect on the viability of Caco-
2 cells.