The aim of this study was to investigate the protective effect of Viburnum opulus L. fruit extract against ischemia/reperfusion (I/R)-induced oxidative stress during the lung transplantation. For this purpose, 30 female rats were firstly randomized to form of donor and recipients. After then, the rats were divided into three groups named as control, I/R, and V. opulus + I/R. Experimental rats were subjected to lung transplantation with ischemia followed by 2 h of reperfusion. Transplantation-related lung injury was evaluated by multiple parameters. A significant decrease was observed in the enzyme activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT), and the levels of glutathione and total antioxidant status (TAS), whereas the levels of malondialdehyde (MDA), total oxidant status (TOS), and protein carbonyl were significantly increased in lung tissue samples of I/R group in comparison to the control group. However, treatment with V. opulus fruit extract resulted in significant reduction of MDA and protein carbonyl levels and increment of the antioxidant system. In conclusion, V. opulus fruit extract showed protective effects against I/R-induced oxidative stress during lung transplantation probably by the radical scavenging and antioxidant activity. Therefore, this fruit extract can be efficient in the prevention of I/R-relate dlung toxicity.