Nanoliposomal Resveratrol as a Novel Approach to Treatment of Diabetes Mellitus

Yücel Ç. , Karatoprak G. , Aktaş Y.

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, vol.18, no.6, pp.3856-3864, 2018 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 18 Issue: 6
  • Publication Date: 2018
  • Doi Number: 10.1166/jnn.2018.15247
  • Page Numbers: pp.3856-3864
  • Keywords: Resveratrol, Diabetes Mellitus, Nanoliposomal Formulations, Oxidative Stress, Pancreatic beta TC Cells, OXIDATIVE STRESS, INSULIN-SECRETION, LOADED LIPOSOMES, ANTIOXIDANT, THERAPY, SYSTEM, CELLS


Diabetes mellitus (DM) is a chronic metabolic disease and the subgroup of DM is called type II which is the most common form. The incidence of type II is increasing worldwide and it focuses on several new approaches to efficiently treatment of diabetes. Resveratrol (RSV) is known to be strong antioxidant and has an insulin-like effect in streptozotocin (STZ)-induced diabetic cells. It plays an active role at treatment of diabetes with reducing the oxidative stress, lowering glucose levels and protection of beta cells which are responsible for insulin secretion. In our study, we prepared two different RSV-loaded nanoliposomes (LPs), characterized in vitro and evaluated efficiencies of LPs on diabetes and related oxidative stress. Release and transport studies of RSV through dialyse membrane and pancreatic beta TC (beta TC) cells were investigated from its solution and LPs. Stability studies were performed at two different conditions (4 degrees C and 25 degrees C +/- 60% relative humidity) for 3 months. Particle size (PS), zeta potential (ZP), polydispersity index (PDI), encapsulation efficiency (EE) and type of the formulations were determined. beta TC cell line was used in cell culture studies and cell viability was measured with using 3-(4,5-dimethyldiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) cytotoxicity test. The antidiabetic effects of RSV LPs were investigated on beta TC cell induced with glucose and STZ and we evaluated relationship between glucose and insulin concentration before and after incubation with LPs containing RSV. Antioxidant and preventive effects of RSV-loaded LPs against diabetes-associated oxidative stress were determined with superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) enzyme assay. When all results were evaluated together, these new developed liposomal formulations significantly decreased high glucose levels in diabetic cell groups synchronous with increasing insulin levels and they showed prolonged antioxidant activity against oxidative stress for 24 hours compared to RSV solution.