Determination of chloramphenicol and tetracycline residues in milk samples by means of nanofiber coated magnetic particles prior to high-performance liquid chromatography-diode array detection

Vuran B., ULUSOY H. İ., SARP G., YILMAZ E., MORGÜL Ü., Kabir A., ...Daha Fazla

Talanta, cilt.230, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 230
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.talanta.2021.122307
  • Dergi Adı: Talanta
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, L'Année philologique, Aerospace Database, Analytical Abstracts, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, EMBASE, Food Science & Technology Abstracts, Linguistic Bibliography, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Anahtar Kelimeler: Chloramphenicol, Tetracycline, HPLC, Carbon nanofiber, Magnetic solid phase extraction, Milk samples
  • Erciyes Üniversitesi Adresli: Evet

Özet

© 2021 Elsevier B.V.A magnetic solid phase extraction (MSPE) coupled with high-performance liquid chromatography-diode array detection (HPLC-DAD) methodology was developed for the determination of chloramphenicol (CP) and tetracycline (TET) antibiotic residues in milk samples. As a solid phase sorbent, C-nanofiber coated magnetic nanoparticles were synthesized and extensively characterized using Field Emission Scanning Electron Microscopy (FE-SEM), Raman Spectroscopy and X-ray Powder Diffraction (XRD) analysis. Experimental variables of MSPE method for both antibiotic analytes were investigated and optimized systematically. After MSPE, the linear range for both the analytes (r2 > 0.9954) were obtained in a range 10.0–600.0 ng mL−1. The limit of detections (LODs) for CP and TET were 3.02 and 3.52 ng mL−1, respectively while RSDs % were below than 4.0%. Finally, the developed method based on MPSE-HPLC-DAD was applied to real milk samples to quantify the antibiotic residues. Recovery values for each antibiotic compound were found in the range of 94.6–105.4% (n = 3) by using spiked model solution.