Aptamer-based impedimetric label-free detection of bisphenol A from water samples using a gold nanoparticle-modified electrochemical nanofilm platform

Saygılı-Canlıdinç R., ÇAĞLAYAN M. O., KARİPER İ. A., Üstündağ Z., ŞAHİN S.

Journal of Applied Electrochemistry, cilt.53, sa.11, ss.2239-2248, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 53 Sayı: 11
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1007/s10800-023-01910-2
  • Dergi Adı: Journal of Applied Electrochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Applied Science & Technology Source, Aquatic Science & Fisheries Abstracts (ASFA), Chemical Abstracts Core, Communication Abstracts, Compendex, Computer & Applied Sciences, INSPEC, Metadex, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.2239-2248
  • Anahtar Kelimeler: Aptamer, Bisphenol A, Gold nanoparticle, Potentiostat/Galvanostat, Sensor
  • Erciyes Üniversitesi Adresli: Evet

Özet

A label-free impedimetric aptasensor for bisphenol A (BPA) was developed based on a novel method. Initially, gold nanoparticle-based nanoplatforms were prepared using a 4-mercaptophenyl (4MP) electrochemical nanofilm. Then, the BPA-selective aptamer (antiBPA) was immobilized on this platform for the sensitive and selective detection of BPA. The characterizations of the electrode preparation steps were performed using a transmission electron microscope, X-ray photoelectron spectroscopy, electrochemical methods, and atomic force microscopy. The aptamer immobilization concentration, immobilization time, and incubation time of BPA were optimized to get the best sensor response. The calibration curve exhibited good linearity between 10 fM and 10 nM with a correlation coefficient of 0.9963 and a limit of detection and limit of quantification values of 8.6 and 25.8 fM (S/N = 3), respectively. The developed aptasensor showed good reproducibility (RSD 2.55%), high sensitivity, specificity, and stability. The developed sensor was validated using real samples of tap water, lake water, and wastewater demonstrating promising performance. Graphical abstract: [Figure not available: see fulltext.].