The objective of this study was to investigate the serum concentrations of iron, manganese, cobalt, copper, zinc, and copper/zinc ratio in Downer cows. The study was performed on 20 recumbent Downer cows and 20 healthy cows. The serum concentrations of iron (P=0.002) and copper (P=0.035) were lower in Downer cows compared with the healthy cows. Serum zinc concentrations, although not significantly (P=0.162), were also lower in Downer cows compared with the healthy cows. Cows with Downer syndrome had greater concentrations of serum AST (P<0.001), LDH (P=0.003), and CK (P<0.001). Other biochemical parameters remained unchanged. RBC (P=0.026), Hb (P=0.029), and MCV (P<0.001) in cows with Downer syndrome had lower values compared with those of healthy ones. However, WBC in cows with Downer syndrome had greater values (P<0.001) compared with those of healthy ones. Other haematological parameters remained similar between Downer cows and healthy cows. It was concluded that serum iron and copper concentrations may play an important role for the aetiology and/or pathogenesis of Downer cows. These elements may be supplemented for the prevention and therapy of recumbency in cows.