Genetic diversity and a long evolutionary history of plum pox virus strain rec in Turkey


Gürcan K., Teber S., Akbulut M., Çağlayan K.

EUROPEAN JOURNAL OF PLANT PATHOLOGY, cilt.161, sa.2, ss.453-461, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 161 Sayı: 2
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1007/s10658-021-02336-2
  • Dergi Adı: EUROPEAN JOURNAL OF PLANT PATHOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, Environment Index, Geobase, Veterinary Science Database
  • Sayfa Sayıları: ss.453-461
  • Anahtar Kelimeler: Genetic diversity center, Recombinant strain, Stone fruits
  • Erciyes Üniversitesi Adresli: Evet

Özet

Plum pox virus strain Recombinant (PPV-Rec) is hypothetically considered as homologous recombinant between strains PPV-M and PPV-D. The nucleotide position 8450 up to end of genome is considered to come from PPV-M, and the remaining major genomic part is PPV-D-derived. It is regarded as third major PPV strain due to its wide distribution and prevalence in Europe. However, among over a thousand PPV isolates genetically identified in Turkey, only 10 of them (<1%) were characterized as PPV-Rec. The nearly complete genome of eight of the PPV-Rec isolates from European part of Turkey were obtained and analyzed together with PPV-Rec isolates of other countries. All major genomic features were conserved among Turkish PPV-Rec isolates. The genetic diversity of PPV-Rec isolates in Turkey (n = 8, 0.015 +/- 0.001%) was found to be comparable to that observed for the isolates from eight countries (n = 10, 0.014 +/- 0.001%). Particularly, genetic diversity of the minor recombinant fragment for Turkish PPV-Rec isolates was apparently higher (0.016 +/- 0.001%) than the value for the 10 isolates from eight countries (0.011 +/- 0.001%). The high genetic diversity was also demonstrated by high phylogenetic variation in Turkish isolates, indicating a long evolutionary history of PPV-Rec isolates in Turkey. After including Turkish isolates in the recombination analysis, the major putative recombination event of PPV-Rec isolates with a breakpoint around position 8450 was identified again, however the other recently reported putative recombination events were not supported in this study by the algorithms implemented in the RDP software.