Phosphorylation of tau protein based on the activity of kinases and phosphatases in various forms of synaptic plasticity

TAN B., Tufan E., Barutçu Ö., Aslan-Gülpinar E., DURSUN N., SÜER C.

Physiology International, cilt.111, sa.1, ss.97-123, 2024 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 111 Sayı: 1
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1556/2060.2024.00344
  • Dergi Adı: Physiology International
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.97-123
  • Anahtar Kelimeler: Akt, LTP/LTD, MAPKs, MAPT gene, metaplasticity, synaptic plasticity, tau protein, tauopathies
  • Erciyes Üniversitesi Adresli: Evet

Özet

The aim of this study is to show the relationship between the change in the strengthening of synaptic plasticity and tau phosphorylation and tau-kinases and phosphatase. The averages of the field excitatory-postsynaptic potential (fEPSP) and population spike (PS) in the last 5 min were used as a measure of LTP, LTD and MP. Total and phosphorylated levels of tau, kinases and phosphatases were evaluated by western blot and mRNA levels were evaluated by RT-qPCR. The stimulation of synapses by HFS and LFSþHFS increased the phosphorylation of total-tau and phospho-tau at the Thr181, Ser202/Thr205, Ser396 and Ser416 residues, and these were accompanied by increased enzymatic activity of Akt, ERK1/2. The increased phosphorylation of tau may mediate maintenance of LTP. If the increase in phosphorylation of tau cannot be prevented, together with inhibition of the subsequent LTP, this may indicate that the physiological role of hyperphosphorylated tau in synaptic plasticity may extend to pathological processes.