Warp and weft directional interlaminar shear strength properties of the developed two dimensional multistitched multilayer E-glass/polyester woven nano composites were studied. When the nano silica material in the unstitched E-glass/polyester composite structure increased, the directional specific interlaminar shear strengths of the unstitched/nano structures increased. On the other hand, the directional specific interlaminar shear strengths of unstitched structures were lower than those of the multistitched/nano structures. All composite structures had interlaminar shear failure between layers in their cross-sections. But, the interlaminar shear failure in multistitched and multistitched/nano structures did not propagate to the large areas. The stitching direction, stitching density, stitching yarn, stitching type and the amount of nano materials in the composite structures were identified as important parameters.