Effects of olive oil-based propolis, caffeic acid phenethyl ester, and methylprednisolone on differentiation of human acute myeloid leukemia cells

DOĞAN H., ÖZÇİMEN A. A., SILICI S.

Journal of Research in Pharmacy, cilt.27, sa.2, ss.677-686, 2023 (ESCI) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 2
  • Basım Tarihi: 2023
  • Doi Numarası: 10.29228/jrp.350
  • Dergi Adı: Journal of Research in Pharmacy
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.677-686
  • Anahtar Kelimeler: Acute myeloid leukemia, Caffeic acid phenethyl ester, differentiation, Metylprednisolon, Olive Oil-Based, Propolis
  • Erciyes Üniversitesi Adresli: Evet

Özet

Leukemia, originating from the hematopoietic system, is a malignant disease for the treatment of which various glucocorticoids are used. The use of natural products alongside conventional therapy has focused on increasing drug-drug interactions and reducing potential side effects. Based on the idea that propolis and caffeic acid phenethyl ester (CAPE) can stimulate cancer cells to differentiation and enhance the effect of chemotherapeutic drugs, we conducted this study. In this study, we investigated whether the synergistic effect of olive oil-based propolis and CAPE is effective with metylprednisolone (MP), which is used for supportive therapy in AML. In the study, OEP (Olive Oil-Based Propolis), CAPE, MP and their combinations were applied to HL-60 cells for 1-3 days. Then, CD11b, CD14 and CD68 antigens were detected on HL-60 cells using flow cytometry techniques to determine the cellular differentiations. As a result, HL-60 cells were found to be significantly directed to CD11b differentiation. OEP and CAPE will enhance the effect of chemotherapy by differentiating cells and ensure treatment completion by minimizing damage to normal tissues and cells.