A cross-sectional study was conducted to determine the prevalence of Fasciola hepatica and to investigate the related risk factors in cattle from Kayseri, Turkey. Faecal and blood samples were collected from 282 cattle from May 2004 to April 2005 and were examined by modified McMaster sedimentation and ELISA techniques to detect Fasciolar sp. eggs and anti-F. hepatica antibodies, respectively. Of the total of 282 cattle, 184 were seropositive for F. hepatica with a prevalence of 65.2%. In addition 24.5% of seropositive cattle had fluke eggs in the faecal examination. The mean number of EPG in infected cattle was 42.8 +/- 4.4. The highest prevalence was observed in >= 6 age group (87.2%) followed by 3-5 (79.5%) and <= d2 age groups (51.6%). The differences between <= 2 and other age groups were found significant (p<0.001), whereas no statistically significant difference (p>0.05) was observed between 3-5 and 6 age groups. The infection was more prevalent in females (70.7%) than males (47.8%) and in cattle from the traditional farms (76.5%) than the small-scale dairy farms (37.2%). No statistically significant difference (p>0.05) was observed related to breed. Our results highlight the importance of initiating a control program for fasciolosis based on regular treatment and prophylaxis in Kayseri Province.