ISOLATION OF DNA BY PHENOL-CHLOROFORM EXTRACTION METHOD FROM SHED AND WAITING FEATHERS OF PSITTACIFORMESBIRD ORDER

Aksel E. G., Kasırga H. İ., Tekin M., Arslan K., Akyüz B.

JOURNAL OF APPLIED BIOLOGICAL SCIENCES, cilt.17, sa.3, ss.460-466, 2023 (TRDizin)

Özet

In this study, it was aimed to isolate DNA from shed and waiting (at least two weeks) wing/tail feathers of the Psittaciformes bird order (Ara chloropterus, Cacatua galerita, Lorius garrulous, Amazona ochrocephala, Ara ararauna, Psittacus erithacus, n=17) using the phenol-chloroform-isoamyl alcohol extraction method. The isolated DNAs was subjected to polymerase chain reaction with a primer commonly used for sex determination in birds. According to the nanodrop measurement results, it was revealed that sufficient DNA concentration to be used in DNA-based molecular studies can be obtained using the phenol-chloroform isolation method. Gender-specific band images were obtained from all DNA samples isolated according to PCR analysis performed for control purposes. As a result, it was revealed that DNA isolation can bedone successfully with the Phenol-chloroform isolation method from the shed and waiting wing/tail feathers of the bird species Psittaciformes. It was concluded that non-invasively obtained DNAs can be used in molecular methods