Comparative efficacy of different sources of platelet-rich plasma on cutaneous wound healing in diabetic rabbit models Eficacia comparativa de diferentes fuentes de plasma rico en plaquetas en la cicatrización de heridas cutáneas en conejos diabéticos
Revista Cientifica de la Facultad de Veterinaria, cilt.36, sa.3, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 36 Sayı: 3
- Basım Tarihi: 2026
- Doi Numarası: 10.52973/rcfcv-e363965
- Dergi Adı: Revista Cientifica de la Facultad de Veterinaria
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, Directory of Open Access Journals
- Anahtar Kelimeler: cicatrización de heridas, conejos diabéticos, diabetic wound healing, histopathology, histopatología, medicina regenerativa, Plasma rico en plaquetas, Platelet-rich plasma, rabbits, regenerative therapy
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Erciyes Üniversitesi Adresli: Evet
Özet
Therapeutic effects of varied-source Platelet-Rich Plasma gel on diabetic rabbit 4 cm2 squared full-thickness cutaneous wounds were assessed. Thirty New-Zealand Rabbits (mean weight: 2.63 ± 0.35 kg; age range: 12–18 weeks) were divided into five groups of six each. Heterologous Platelet-Rich Plasma was sourced from a male Akkaraman sheep (age: 1 year; weight: 50 kg). Diabetes induction: Alloxan monohydrate (100 mg/kg, 2 doses, 48h apart). Regulation: Recombinant DNA human insulin. Baseline blood glucose: 100.5 ± 6.5 mg/dL; post-alloxan (5d): 336.9 ± 51.9 mg/dL (P = 0.000). Double centrifugation and CaCl2 gelation produced PRP with 3.5 times baseline platelet concentration. Post-wounding clinical parameters, morphometric and morphologic evaluations were done at 0th, 3rd, 7th, 10th, 14th, 17th, 21st, and 25th days. Histopathology was performed on day 25. Acceleration in wound healing was observed in all Platelet-Rich Plasma treated (i.e. Diabetic Autologous-Platelet-Rich Plasma, Diabetic Homologous-Platelet-Rich Plasma, Diabetic Heterologous-Platelet-Rich Plasma) groups, meanwhile in both control groups, delayed wound healing was detected, especially in the Diabetic Rabbit Normal Saline group. Enhanced wound contraction observed in all Platelet-Rich Plasma groups (60% by day 14th) vs. controls (Healthy Rabbit Normal Saline & Diabetic Rabbit Normal Saline: 56% on days 14th & 17th respectively). These values later on day 21st and day 25th reached up to 95.72 ± 3.74% and 99.23 ± 0.71% for Diabetic Autologous-Platelet-Rich Plasm; 92.44 ± 5.07% and 96.04 ± 3.70% for Diabetic Homologous-Platelet-Rich Plasm; 89.37 ± 12.24% and 97.32 ± 3.12% for Diabetic Heterologous-Platelet-Rich Plasm; while 76.96 ± 15.04% and 84.33 ± 9.29% for Diabetic Rabbit Normal Saline and 84.91 ± 17.30% and 92.27 ± 14.72% for Healthy Rabbit Normal Saline; (P = 0.036 & P = 0.019 respectively). Histopathologically Platelet-Rich Plasma augmented the wound healing cascade, with early angiogenesis, healthy granulation, and sufficient re-epithelialization. Regardless of the Platelet-Rich Plasma source, it modulates and accelerates cutaneous wound healing in diabetic rabbit models, highlighting its potential as a valuable adjunct therapy for wound healing in diabetic patients.