Molecular characterization and association of ISSR markers with some fruit properties of Morus alba L. genotypes in Southeastern Türkiye

DÖNMEZ D., Karaat F. E., AKA KAÇAR Y., ŞİMŞEK Ö.

Genetic Resources and Crop Evolution, cilt.70, sa.7, ss.2129-2142, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 70 Sayı: 7
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1007/s10722-023-01563-2
  • Dergi Adı: Genetic Resources and Crop Evolution
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Geobase, Veterinary Science Database
  • Sayfa Sayıları: ss.2129-2142
  • Anahtar Kelimeler: Characterization, Fruit, ISSR, Molecular markers, Morus alba L
  • Erciyes Üniversitesi Adresli: Evet

Özet

Mulberry is a preferred fruit, especially with its high antioxidant capacity, rich nutritional value, and unique aroma due to its high phenolic compound content. In recent years, the demand for functional foods, whose contribution to human health is better understood, has increased the demand for mulberry fruits. Türkiye has significant potential in terms of mulberry genetic resources. However, these genetic resources have not been sufficiently investigated. The present study investigated genetic relationships and fruit characteristics of mulberries collected from Adıyaman province in the Southeastern region of Türkiye. A total of 68 mulberry genotypes were evaluated. Fresh fruit weight (FFWE), fresh fruit length (FFL), fresh fruit width (FFWI), dry fruit yield (DFY), length of the peduncle (LOP), and total soluble solids (TSS) of fruits were measured and evaluated. In addition, Spearman’s Correlation Test conducted a correlation between all the obtained fruit quality and molecular marker characterization results. ISSR markers were used to determine genetic relationships. According to the results of fruit quality characteristics, the highest FFWE, FFL, FFWI, DFY, LOP, and TSS were observed in Adıyaman Centrum-8, Gölbaşı-2, Adıyaman Centrum-5, 8, Kahta-8, Kahta-2, and Ulukale genotypes, respectively. Adıyaman Centrum 5–8, Kahta-2–8, Gölbaşı-2, and Ulukale were promising genotypes regarding fruit quality characteristics. The genetic similarity ratio ranged from 0.51 to 0.96. Clustering based on Jaccard’s similarity coefficient and a UPGMA revealed two main clusters. Samsat-4, Çelikhan-5, Adıyaman Centrum-5, Adıyaman Centrum-8, Besni-2, and Besni-5 genotypes were separated from all other genotypes and clustered in the first main branch. All other genotypes were clustered in the second main branch. Potential markers for early selection were determined by correlating fruit characteristics with ISSR DNA profiles.