The application of plant growth-promoting rhizobacteria (PGPRs) can be an excellent and eco-friendly alternative to the use of chemical fertilizers. While PGPRs are often used in traditional agriculture to facilitate yield increases, their use in soilless agriculture has been limited. Soilless agriculture is growing in popularity among commercial farmers because it eliminates soil-borne problems, and the essential strategy is to keep the system as clean as possible. However, a new trend is the inclusion of PGPRs to enhance plant development. Despite the plethora of research that has been performed to date, there remains a huge knowledge gap that needs to be addressed to facilitate the commercialization of PGPRs for sustainable soilless agriculture. Hence, the development of proper strategies and additional research and trials are required. The present review provides an update on recent developments in the use of PGPRs in soilless agriculture, examining these bacteria from different perspectives in an attempt to generate critical discussion and aid in the understanding of the interaction between soilless agriculture and PGPRs.