Construction of genetic linkage map for Ficus carica L. based on AFLP, SSR, and SRAP markers


İKTEN H., Sevinc Solak S., GÜLŞEN O., MUTLU N., İKTEN C.

HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY, cilt.60, sa.5, ss.701-709, 2019 (SCI-Expanded) identifier identifier

Özet

A new genetic linkage map of Ficus carica (2n=2x=26) was constructed using 149 F1 progeny derived from the cross between two fig cultivars, 'Bursa Siyah' (BS) and 'Ak Ilek' (AK). Fifty-two amplified fragment length polymorphism, 49 simple sequence repeat (SSR), 16 sequence-related amplified polymorphism (SRAP), and 12 sequence characterized amplified region (SCAR-SRAP) combinations were used to generate markers for the map. The BS map consisted of 229 markers, distributed to 16 linkage groups (LGs), with an average marker density of 5.98 cM and map distance of 1342 cM. The AK linkage map carried 244 markers, distributed to 16 LGs, with an average marker density of 4.90 cM and map distance of 1191 cM. The consensus map comprises 355 markers, 1474 cM in length, with average marker density of 4.15 cM. The map indicates the location of new SSRs, nine of which were transferred from related species, and might be helpful for mapping quantitative trait loci that control important horticultural traits in the future.