MOLECULAR CHARACTERIZATION OF SOME SELECTED PERSIMMON GENOTYPES AND CULTIVARS BY SRAP AND SSR MARKERS

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PINAR H., YILDIZ E., KAPLANKIRAN M., TOPLU C., ÜNLÜ M., SERÇE S., ...Daha Fazla

GENETIKA-BELGRADE, cilt.49, sa.2, ss.693-704, 2017 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 49 Sayı: 2
  • Basım Tarihi: 2017
  • Doi Numarası: 10.2298/gensr1702693p
  • Dergi Adı: GENETIKA-BELGRADE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.693-704
  • Anahtar Kelimeler: Persimmon, selection, genetic diversity, molecular markers, DIOSPYROS-KAKI THUNB., GENETIC DIVERSITY ANALYSIS, PHYLOGENETIC RELATIONSHIP, SPP. EBENACEAE, RFLP ANALYSIS, RAPD, L., IDENTIFICATION, INDUSTRY, JAPANESE
  • Erciyes Üniversitesi Adresli: Evet

Özet

In this study, SRAP and SSR markers were employed to determine genetic relationships among 42 persimmon genotypes (Diospyros kaki Thunb) obtained from Hatay province and 3 persimmon cultivars, 2 of which belong to Diospyros kaki Thunb and one belongs to Diospyros oleifera Cheng. Genetic relationships were determined by using a total of 29 molecular DNA primers (SRAP and SSR). Of these primers, 21 SRAP primer combinations produced a total of 107 bands and 77.6% of them were polymorphic; 8 SSR primers produced 26 polymorphic bands with an average polymorphism ratio of 84.6%. The SRAP and SSR markers produced 4.6 bands as average and the number of bands produced per marker was calculated as 3.6. The lowest similarity was observed between MK-113 (Diospyros oleifera Cheng) and the other genotypes all belongs to Diospyros kaki Thunb (with similarity ratios of 0.41-0.69 for SRAP primers, between 0.25-0.67 for SSR primers). The genotypes/cultivars belongs to Diospyros kaki had similarity ratio between 0.98-1.00 according to SRAP and SSR markers. This synonym or similarity could be results of clonal propagation rather than autogamy.