Molecular discrimination of Echinococcus granulosus and Echinococcus multilocularis by sequencing and a new PCR-RFLP method with the potential use for other Echinococcus species


Sakalar C., Kuk S., Erensoy A., Dagli A. F. , Ozercan I. H. , ÇETİNKAYA Ü. , ...Daha Fazla

TURKISH JOURNAL OF MEDICAL SCIENCES, cilt.44, ss.741-748, 2014 (SCI İndekslerine Giren Dergi) identifier

  • Cilt numarası: 44 Konu: 5
  • Basım Tarihi: 2014
  • Doi Numarası: 10.3906/sag-1303-114
  • Dergi Adı: TURKISH JOURNAL OF MEDICAL SCIENCES
  • Sayfa Sayıları: ss.741-748

Özet

Background/aim: To develop a novel polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) protocol using a new genomic marker sequence and a novel set of restriction enzymes in order to detect and discriminate 2 Echinococcus species, E. granulosus and E. multilocularis, found in formalin-fixed paraffin-embedded (FFPE) human tissues.