Molecular discrimination of Echinococcus granulosus and Echinococcus multilocularis by sequencing and a new PCR-RFLP method with the potential use for other Echinococcus species


Sakalar C., Kuk S., Erensoy A., Dagli A. F., Ozercan I. H., ÇETİNKAYA Ü., ...More

TURKISH JOURNAL OF MEDICAL SCIENCES, vol.44, no.5, pp.741-748, 2014 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 5
  • Publication Date: 2014
  • Doi Number: 10.3906/sag-1303-114
  • Journal Name: TURKISH JOURNAL OF MEDICAL SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.741-748
  • Erciyes University Affiliated: Yes

Abstract

Background/aim: To develop a novel polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) protocol using a new genomic marker sequence and a novel set of restriction enzymes in order to detect and discriminate 2 Echinococcus species, E. granulosus and E. multilocularis, found in formalin-fixed paraffin-embedded (FFPE) human tissues.