Molecular discrimination of Echinococcus granulosus and Echinococcus multilocularis by sequencing and a new PCR-RFLP method with the potential use for other Echinococcus species

Sakalar, Çağrı; Kuk, Salih; Erensoy, Ahmet; Dagli, Adile; Ozercan, Ibrahim; ÇETİNKAYA, ÜLFET; Yazar, Süleyman

doi:10.3906/sag-1303-114

Molecular discrimination of Echinococcus granulosus and Echinococcus multilocularis by sequencing and a new PCR-RFLP method with the potential use for other Echinococcus species

Sakalar C., Kuk S., Erensoy A., Dagli A. F., Ozercan I. H., ÇETİNKAYA Ü., ...Daha Fazla

TURKISH JOURNAL OF MEDICAL SCIENCES, cilt.44, sa.5, ss.741-748, 2014 (SCI-Expanded, Scopus, TRDizin)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 44 Sayı: 5
Basım Tarihi: 2014
Doi Numarası: 10.3906/sag-1303-114
Dergi Adı: TURKISH JOURNAL OF MEDICAL SCIENCES
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
Sayfa Sayıları: ss.741-748
Anahtar Kelimeler: FFPE tissues, Echinococcus granulosus, Echinococcus multilocularis, sequencing, PCR-RFLP
Erciyes Üniversitesi Adresli: Evet

Özet

Background/aim: To develop a novel polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) protocol using a new genomic marker sequence and a novel set of restriction enzymes in order to detect and discriminate 2 Echinococcus species, E. granulosus and E. multilocularis, found in formalin-fixed paraffin-embedded (FFPE) human tissues.