Molecular discrimination of Echinococcus granulosus and Echinococcus multilocularis by sequencing and a new PCR-RFLP method with the potential use for other Echinococcus species

Sakalar, Çağrı; Kuk, Salih; Erensoy, Ahmet; Dagli, Adile; Ozercan, Ibrahim; ÇETİNKAYA, ÜLFET; Yazar, Süleyman

doi:10.3906/sag-1303-114

Molecular discrimination of Echinococcus granulosus and Echinococcus multilocularis by sequencing and a new PCR-RFLP method with the potential use for other Echinococcus species

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Sakalar C., Kuk S., Erensoy A., Dagli A. F., Ozercan I. H., ÇETİNKAYA Ü., ...More

TURKISH JOURNAL OF MEDICAL SCIENCES, vol.44, no.5, pp.741-748, 2014 (SCI-Expanded)

Publication Type: Article / Article
Volume: 44 Issue: 5
Publication Date: 2014
Doi Number: 10.3906/sag-1303-114
Journal Name: TURKISH JOURNAL OF MEDICAL SCIENCES
Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
Page Numbers: pp.741-748
Erciyes University Affiliated: Yes

Abstract

Background/aim: To develop a novel polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) protocol using a new genomic marker sequence and a novel set of restriction enzymes in order to detect and discriminate 2 Echinococcus species, E. granulosus and E. multilocularis, found in formalin-fixed paraffin-embedded (FFPE) human tissues.