Akademik Veri Yönetim Sistemi
BIOLOGIA, cilt.81, sa.2, 2026 (SCI-Expanded, Scopus)
Chrysanthemum, a member of the Asteraceae family, is an important ornamental plant cultivated worldwide. Despite being propagated by green cuttings, plant losses occur during vegetative propagation. To enhance root formation and ensure high-quality root development in chrysanthemums propagated by cuttings, plant growth-promoting rhizobacteria (PGPR) applications are utilized. In this study, the effect of different PGPR bacterial formulations, whose presence of the IAA (indole acetic acid) gene was detected through PCR (polymerase chain reaction) analyses, on the rooting performance of garden-type chrysanthemum cuttings was investigated. Rhizobacteria with the presence of the IAA gene was activated, and three different bacterial formulations were prepared: Formulation 1 (a mixture of Bacillus subtilis and Bacillus megaterium), Formulation 2 (Bacillus cereus), and Formulation 3 (a mixture of Pseudomonas putida and Stenotrophomonas maltophilia). These formulations were applied to chrysanthemum green cuttings by immersion. The results revealed that rhizobacteria applications had positive effects on rooting and seedling quality of chrysanthemum green cuttings. Significant increases were observed in rooting rate, root length, seedling height, seedling leaf number, and seedling fresh weight compared to the control group. Rhizobacteria applications from different species resulted in a 40-50% increase in green cutting rooting rate compared to the control, while approximately a two-fold increase in seedling height, leaf number, and seedling fresh weight was achieved compared to the control, supporting seedling root development. Additionally, protein isolation from leaves taken from cuttings revealed positive effects on morphological characteristics as well as protein levels. In conclusion, the findings indicate that rhizobacteria from different species have significant positive effects on rooting, seedling quality, and protein profile in ornamental plants.