Cytotoxic Activity of Pimpinella isaurica and In Silico Analysis of Flavonoids
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, vol.32, no.4, pp.657-662, 2022 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 32 Issue: 4
- Publication Date: 2022
- Doi Number: 10.1007/s43450-022-00289-z
- Journal Name: REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, EMBASE, International Pharmaceutical Abstracts, Veterinary Science Database
- Page Numbers: pp.657-662
- Keywords: Cancer, Antiproliferative activity, MTT assay, Flavonoids, Apigenin-7-O-glucoside, Luteolin-7-O-glucoside
- Erciyes University Affiliated: Yes
Abstract
Pimpinella L., Apiaceae, is one of the largest genera and has a broad spectrum of biological or pharmacological activities including antiproliferative effects against cancer cells. In the present study, the cytotoxic activity of the methanol extracts and sub-extracts of Pimpinella isaurica V.A. Matthews subsp. isaurica was investigated against the cervical epithelial carcinoma cell line (HeLa), the liver carcinoma cell line (HepG2), the breast carcinoma cell line (MCF-7), and the lung carcinoma cell line (A549) by MTT assay. For the chemical profiling, the methanol extract was analyzed for phenolic acids and flavonoids by HPLC-DAD. Mutagenic and biological activities of detected compounds were predicted with in silica methods. P. isaurica subsp. isaurica methanol extract showed potent activity on all tested cell lines according to National Cancer Institute guidelines (IC50 ranges 5.36-15.57 mu g/ml). Apigenin-7-O-glucoside (0.098 g/100 g) and luteolin-7-O-glucoside (0.078 g/100 g) were revealed in the most active extract, and these compounds were predicted as nonmutagenic. The cytotoxic activity of P. isaurica subsp. isaurica against cervical, liver, breast, and lung cancer cells and flavonoids in the plant was reported for the first time.