Triple-combination therapy against cisplatin-resistant ovarian cancer cells


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Kip B., Aydın Ö.

International Congress on Biological and Health Sciences, Afyonkarahisar, Turkey, 26 - 28 February 2021, pp.169

  • Publication Type: Conference Paper / Summary Text
  • City: Afyonkarahisar
  • Country: Turkey
  • Page Numbers: pp.169
  • Erciyes University Affiliated: Yes

Abstract

Abstract: Cisplatin resistance is an important problem in ovarian cancer. Drug resistance develops in different ways such as efflux, influx, drug detoxification, DNA repair, and apoptosis failure. The existence of different resistance-building mechanisms indicates that a strategy targeting a particular pathway may not produce an efficient sensitization to the drug. Therefore, more effective treatments are needed in cancer cells that have acquired drug resistance. Ultrasound has a wide range of applications in medicine and is widely used in both diagnosis and treatment. Exposing biological systems to low-intensity ultrasonic waves causes mechanical and thermal effects known as biological effects. Ultrasound waves cause thermal/mechanic effects leading to increases cell membrane permeability. The combined effect of low-intensity ultrasound and chemotherapy is a promising method in anti-cancer therapy. With the increased membrane porosity in the presence of ultrasound energy, more chemotherapy agents are allowed to enter the cells. Solid cavitation agents can be used to increase the effectiveness of ultrasound therapy because they do not deform quickly in the presence of acoustic energy. Among these agents, gold nanocones (AuNCs) synthesized by the oil-inwater emulsion method attract attention with their large, rough surface areas and biocompatibility. In this study, we developed ultrasound active AuNCs and treated the cisplatin sensitive ovarian cancer cells (A2780) and cisplatin resistance ovarian cancer cells (A2780cis) with therapeutic ultrasound (TUS)+AuNCs+cisplatin to overcome drug resistance. When both cell lines were treated with the calculated IC50 values of cisplatin, AuNCs, and TUS, less than 20% cell viability was obtained. Our proposed treatment strategy was statistically significant and effective compared to the other treatment groups. The results show promise in the development of effective therapy against cisplatin-resistant cells. Keywords: therapeutic ultrasound, gold nanocone, cisplatin resistance, ovarian cancer This work has been supported by Erciyes University Scientific Research Projects Coordination Unit under grant number 10657.