This study was conducted to determine DNA-polymorphism of a SacII RFLP at exon 7 of the beta-lactoglobulin (beta-LG) gene in Honamli (31), Hair (39) and Saanen (41) goat breeds. A total of 111 goats were genotyped for the beta-lactoglobulin-SacII polymorphism by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). In the studied breeds, digestion of amplification product with SacII restriction enzyme revealed two alleles namely, A and B and three genotypes (AA, BB and AB). Allelic frequencies for Hair, Saanen and Honamli breeds were 0.42, 0.37 and 0.53 respectively for A allele; 0.58, 0.63 and 0.47 respectively for B allele, while genotypic frequencies were 0.13, 0.12 and 0.19 for AA, 0.28, 0.39 and 0.13 for BB, and 0.59, 0.49 and 0.68 for AB respectively. Deviation from Hardy-Weinberg equilibrium was not observed in the studied breeds. As a result, this study provided information on the polymorphism of beta-lactoglobulin in three goat breeds. Additionally, this study reported the existence of a genetic polymorphism at beta-LG gene in Honamli goat breed for the first time.