A sensitive spectrophotometric ellipsometry based Aptasensor for the vascular endothelial growth factor detection


KARİPER İ. A. , Üstündağ Z., Caglayan M. O.

Talanta, vol.225, 2021 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 225
  • Publication Date: 2021
  • Doi Number: 10.1016/j.talanta.2020.121982
  • Title of Journal : Talanta
  • Keywords: Aptamers, Biosensors, Breast cancer, Spectroscopic ellipsometry, VEGF, RESONANCE ENERGY-TRANSFER, ROLLING CIRCLE AMPLIFICATION, PROSTATE-SPECIFIC ANTIGEN, FACTOR VEGF, LABEL-FREE, SPECTROSCOPIC ELLIPSOMETRY, PROGNOSTIC INDICATOR, SYSTEMATIC EVOLUTION, SELECTIVE DETECTION, DYNAMIC-RANGE

Abstract

© 2020 Elsevier B.V.A sensitive and selective, aptamer and spectroscopic ellipsometry based sensor is reported here for the early diagnosis of breast cancer, which is a common type of cancer following lung cancer. It was aimed to develop a single-step and label-free assay for the sensitive and selective detection of VEGF165. To this end, two different aptamers and spectroscopic ellipsometry were used. In the presented study, by determining the appropriate aptamer immobilization conditions, the spectroscopic ellipsometry technique was successfully applied for the detection of VEGF165 at the range of 1 pM–1000 pM in the buffer. Aptasensors have a detection limit of 5.81 pM and 4.29 pM, respectively.