Catalase/Fe3O4@Cu2+ hybrid biocatalytic nanoflowers fabrication and efficiency in the reduction of organic pollutants

Alhayali N. I., Özpozan N., Dayan S., Özdemir N., Yilmaz B.

POLYHEDRON, vol.194, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 194
  • Publication Date: 2021
  • Doi Number: 10.1016/j.poly.2020.114888
  • Journal Name: POLYHEDRON
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chemical Abstracts Core
  • Keywords: Fe3O4, Hybrid nanoflowers, Catalase, Reduction of nitrobenzenes
  • Erciyes University Affiliated: Yes


In this work, the magnetic spinel-Fe nanomaterials (Fe3O4 NMs) were prepared, and then the catalase was immobilized to the Fe3O4. After, the enzyme@inorganic hybrid nanoflowers (catalase/Fe3O4@Cu2+ hNFs) were synthesized using the catalase as the organic component and Cu2+ ions and Fe3O4 as the inorganic components, defined as a catalyst. The catalase/Fe3O4@Cu2+ hNFs were characterized by using various techniques such as FT-IR, SEM, EDX, as well as XRD. The fabricated catalase/Fe3O4@Cu2+ hNFs at different pH (6.0, 7.4, 8.0, and 9.0) were tested in the reduction of nitrobenzenes and organic dye. The best results for catalase/Fe3O4@Cu2+ hNFs at different pHs were found to be 77.61%, 68.60%, 82.95%, and 91.52% with 4-nitroaniline, respectively. Herein, the effect of both pH and substrate change on the conversion of the catalytic reaction was examined. (C) 2020 Elsevier Ltd. All rights reserved.