The effect of row space (RS) and irrigation (IR) on total protein, total oil, and fatty acid composition of Harran-grown sesame seed was studied. Total oil content of sesame varied from 46.4 to 51.5%. The oil and protein contents were significantly different among treatments (P < 0.01) in 1998 and 1999. IR affected oil content significantly (P < 0.01) in both years, and RS had no significant effect. The protein content was significantly influenced by RS and IR at P < 0.05 and P < 0.01, respectively. RS 70 cm had the highest protein content followed by RS 60, 50, and 40 cm, respectively. IR every 24th day resulted in the highest level of protein, followed by 18th-, 12th-, and 6th-day irrigation, respectively. Correlation coefficients between protein and oil content were -0.34 in 1998 and -0.59 in 1999. RS (P < 0.05) and IR (P < 0.01) influenced oleic and linoleic acid contents significantly. Interactions of RS and IR were also found to be significant (P < 0.05) over the oleic and linoleic acid levels.