Akademik Veri Yönetim Sistemi
MICROCHEMICAL JOURNAL, cilt.218, 2025 (SCI-Expanded, Scopus)
Background: Amino acids play critical roles in various biological and metabolic processes. Accordingly, accurate determination of their levels is essential in clinical diagnosis. Although traditional blood collection techniques are commonly used in amino acid analysis, microscale blood sampling methods offer a promising alternative. Aim: In this study, the effectiveness of microscale volumetric absorptive sampling, namely MAS-10 (R), in amino acid analysis is evaluated using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methods: The validity of the process is evaluated following the ICH Q2 (R1) guidelines, and satisfactory results are obtained in terms of accuracy, precision, and reproducibility. Results: The calibration curves exhibit strong linearity in the tested concentration ranges (R-2 > 0.995). Fisher's F test demonstrated variance homogeneity across concentration levels for all amino acids (p > 0.05), confirming the robustness and consistency of the calibration model. Sensitivity analyses confirm that the method is suitable for determining trace levels of amino acids with low detection and assay limits. Significantly, recovery rates consistently fall within the 95-117 % range, underscoring the method's reliability for routine applications and instilling confidence in its use. Conclusions: The results affirm that MAS-10 (R) can be an alternative to traditional venous blood collection methods. It offers a significant advantage, especially for newborns, oncology patients, and other patient groups requiring a low volume of samples. Due to its compatibility with LC-MS/MS and its high accuracy and sensitivity, MAS-10 (R) appears to be a promising tool for metabolomics analysis, clinical diagnostics, and biochemical research.