Dynamic monitoring of the cytotoxic effects of Cousinia iconica extracts on A549 and RL95-2 cells using the xCELLigence RTCA system

PASHAYEVA L., Arslan A. K. K., DEMİRPOLAT E., Tugay O.

Indian Journal of Traditional Knowledge, cilt.23, sa.6, ss.569-573, 2024 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 23 Sayı: 6
  • Basım Tarihi: 2024
  • Doi Numarası: 10.56042/ijtk.v23i6.11827
  • Dergi Adı: Indian Journal of Traditional Knowledge
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.569-573
  • Anahtar Kelimeler: Asteraceae, Cytotoxicity, RL95-2, xCELLigence
  • Erciyes Üniversitesi Adresli: Evet

Özet

Asteraceae family plants have potential anticancer activity. In this study, the cytotoxic effects of C. iconica were investigated using different tumor cell lines: Non-small lung cancer (A549) and human endometrium carcinoma cell line (RL95-2). The cytotoxicity of the extracts was determined with MTT assay and the xCELLigence Real-Time Cell Analyser (RTCA). As a result, the ethyl acetate sub-extract and methanol extract of C. iconica were found cytotoxic to RL95-2 cell line with 271 and 51 µg/mL IC50 values, respectively. According to RTCA results, the IC50 values of ethyl acetate sub-extract were 44.82, 60.89, and 55.22 µg/mL and methanol extract 265.52, 250.48, and 246.65 µg/mL, for 24, 48, and 72 h exposure, respectively. RL95-2 cells are more sensitive to ethyl acetate sub-extract than methanol extract. This study is the known first study to show the cytotoxic effects of C. iconica extracts on both A549 and RL95-2 cell lines.