In the present study, the biochemical composition and shape and dimensional traits of 25 rosehip (Rosa canina) genotypes were investigated. The shape and dimensional traits were determined by image processing technique. Seed-propagated rosehip genotypes belonging to R. canina were collected from the natural flora of Mesudiye (Ordu) and Talas (Kayseri) districts. Antioxidant activity (39.510-72.673 mmol center dot kg(-1)), total flavonoids (287.80-1,686.20 mg quercetin equivalent (QE) center dot kg(-1)) and total phenolics (38,519.40-79,080.60 mg gallic acid equivalent center dot kg(-1)) of the genotypes exhibited large variations. Width (12.2 mm) and thickness (12.5 mm) of fruits averages were found to be close to each other. The genotypes exhibited fruit lengths between 12.0 mm and 29.5 mm. Average projected area at horizontal orientation (179.7 mm(2)) was greater than the projected area at vertical orientation (120.4 mm(2)). Sphericity average was calculated as 71.4%. According to principal component (PC) analysis, the most important dimensional traits discriminating genotypes from each other were identified as surface area, geometric mean diameter and volume. In terms of shape attributes, distinctive differences were observed in sphericity, circularity, elongation and surface closure rates (SCR) of the genotypes. According to elliptic Fourier analysis (EFA), genotypes look like a sphere. In terms of shape, there were long, spherical, flat bottomed, pointed bottomed and asymmetric-looking genotypes indicating how environment and genotype affect the fruit shape. The greatest shape variation was transverse contraction and expansion. According to the clustering analysis for shape attributes, rosehip genotypes were classified into six groups. Dendrogram, scatter plots of linear discriminant analysis and paired comparison test results put forth the shape differences of the genotype successfully.