IN VITRO COMPARISON OF THE EFFECTS OF DENTAL FILLING MATERIALS ON MOUSE FIBROBLASTS


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kılıç D., Kesim S., LİMAN N., Sumer Z., ÖZTÜRK A.

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, vol.26, no.4, pp.3155-3162, 2012 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 26 Issue: 4
  • Publication Date: 2012
  • Doi Number: 10.5504/bbeq.2012.0030
  • Journal Name: BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.3155-3162
  • Keywords: fibroblast, MTT, cytotoxity, glass ionomer cement, cell morphology, GLASS-IONOMER CEMENTS, HUMAN GINGIVAL FIBROBLASTS, CORONALLY POSITIONED FLAP, INVITRO ASSESSMENT, HUMAN OSTEOBLASTS, MILLIPORE FILTER, COMPOSITE RESINS, CYTO-TOXICITY, ROOT SURFACE, CELL-LINES
  • Erciyes University Affiliated: Yes

Abstract

The choice of filling material is an important factor in the clinical success of root coverage. Therefore, the cytotoxicity of filling materials must be investigated to ensure a safe biological response. The aim of this study was to compare the response of L929 mouse fibroblasts to several glass ionomer cements (GICs), i.e. conventional GIC, resin-modified glass ionomer cement (RMGIC) and polyacid-modified resin composite (PMRC), using three different methods. 1) 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay 2) agar diffusion test, 3) scanning electron microscopy