By 2050, it is predicted that biofuels will provide 27% of total transport fuel and avoid around 2.1 Gt CO2emissions per year when produced sustainably. Biodiesel is a renewable fuel that can be produced by trans-esterification in any climate using already developed agricultural practices. This paper aims to study various physical and chemical properties of biodiesel synthesized from edible and non-edible feedstocks. These feedstocks include Croton megalocarpus, Calophyllum inophyllum, Moringa (Moringa oleifera), palm (Elaeis guineensis) and coconut (Cocos nucifera). The physical and chemical properties of biodiesel–diesel blends were also presented. Furthermore, the regression analysis method was used to predict the properties of biodiesel blends. It has been found that blending of diesel with biodiesel has resulted in much improvement in kinematic viscosity, density, calorific value and oxidation stability. However, flash point and viscosity index decrease as the percentage of diesel increases. Moreover, the mathematical relationships developed in this study show a high regression values (R2) between properties and biodiesel–diesel blends. As a conclusion, it is believed that the outcome of this study gives the readers valuable results that help to predict the properties of biodiesel and its blends with diesel which are substantial parameters in the design of fuel system for biodiesel engine.