Development of in situ gel formulation containing bisphosphonate-loaded PLGA microspheres for bone regeneration in maxillofacial surgery applications; Formulation, in vitro characterization and release kinetic studies

Polat H. K., Ünal S.

Journal of Faculty of Pharmacy of Ankara University, cilt.46, sa.3, ss.993-1008, 2022 (Scopus)

Özet

        Objective: In this study, it was aimed to locally apply the bisphosphonate-loaded microsphere drug delivery system with in situ gel formulation, which was prepared to increase bone regeneration in the implant area in maxillofacial surgery.

        Material and Method: In order to design the combination delivery system, bisphosphonate-loaded PLGA microspheres were embedded in the prepared in situ gel formulations. In vitro drug release, pH, clarity, sol-gel transition temperature and release kinetic studies were all assessed for the developed formulations.

        Result and Discussion: The produced formulations' in situ gelation temperatures ranged from 33 to 37°C; their pH values were in the range of 6; and they were all syringeable, which is defined as the force required to expel each formulation from a syringe equipped with a 20-gauge needle. With the preparations, the amounts of P407 and chitosan increased, lowering in vitro burst release while simultaneously raising viscosity. However, each in situ gel formulation releases over a period of 14 days. Consequently, Bisphosphonate loaded PLGA microspheres embedded in situ gel formulations were elucidated in detail and presented as a locally applicable drug delivery system in maxillofacial surgery, especially in dental implant applications