Akademik Veri Yönetim Sistemi
Mediterranean International Conference on Research in Applied Sciences, Antalya, Türkiye, 22 - 24 Nisan 2022, ss.1-3
Abstract
In order to treat the missing teeth in the jaws with dental implants, adequate amount and quality of bone tissue is needed in the maxillo-mandibular area. In order for the treatment to be successful and to function in the mouth for a long time, alveolar bone must be adequate size. Extraction of the teeth initiates the resorption process in the alveolar bone and causes a decrease in the height and/or width of the existing bone. This study was carried out todetermine the effect of methacrylate gelatin (Gel-MA), nanoHydroxyapatite (nHAp) and mesenchymal stem cell (MSCs) systems placed in tooth extraction sockets of rats on 3D bone healing and regeneration. These systems, which are used for bone regeneration, are easy to apply and biocompatible. It has also osteoconductive and osteoinductive properties. Thirty-six Sprague-Dawley rats were randomly assigned to control/blank defect (group I, n=12), Gel- MA+nHAp (group II, n=12) and Gel-MA+ nHAp+MSCs (group III, n=12) in the study. n=12) were divided into 3 experimental groups. Immediately after the extraction of the left maxillary 1st and 2nd molars of the rats, Gel-MA+ nHAp and Gel-MA+nHAp+MSCs were placed in the extraction sockets of the rats in Group II and Group III, respectively. Hydrogel form was created by applying UV light (395-480nm, 30 sec) through the mouth on the placed biomaterials. Half of the animals in all groups were sacrificed at the 4th week and the other half at the 8th week, and tissue samples were taken from the maxilla. Macroscopic and microtomographic (micro-CT) evaluation was performed. According to the results of micro- CT analysis taken from the 4th and 8th week samples of Group III, bone volume percent (BVP) was statistically significant and had the highest value compared to the other groups. In the 4th week micro-CT analysis of Group III, the mean BVP was 81.02±3.05%, and the 8th week mean BVP value was 85.86±1.74% (p <0.001). According to the results of micro-CT analysis taken from tissue samples at the 4th week, a significant difference was found between group I and group III and between group II and group III (p<0.001, p<0.01). Statistically similar differences were found between the same groups at week 8 (p<0.001, p<0.05). In conclusion, it has been shown that Gel-MA+nHAp+MSCs systems are highly effective in 3D bone reconstruction in jaw bones. As a result, it was determined that Gel- MA+nHAp+MSCs systems are very effective in 3D bone reconstruction in jaw bones.
Keywords: Alveolar bone, resorption, mesenchymal stem cell, methacrylated gelatin, nanoHydroxyapatite, reconstruction.