A novel and green deep eutectic solvent based liquid phase microextraction (DES-LPME) methodology has been proposed for the assessment of rhodamine B from cosmetic products and water samples. A deep eutectic solvent (DES) consist of tetrabutyl ammonium chloride-decanoic acid (1:2) as extraction solvent and tetrahydrofuran as emulsification agent were used for the microextraction of rhodamine B. The quantitative recoveries were achieved at pH 3 by using 03 mL of DES and 03 mL of THF. The rhodamine B concentration in last volume was analyzed by mirco-cuvette UV-VIS spectrophotometer at 550 nm. The limit of detection (LOD), limit of quantification (LOQ), preconcentration factor (PF) and relative standard deviation (RSD %) were found as 2.2 mu gL(-1), 7.3 mu gL(-1), 25 and 23%, respectively. Accuracy and validity of the developed method was verified by addition-recovery studies for water and cosmetic samples. (C) 2018 Elsevier B.V. All rights reserved.