In this case report was aimed to the evaluation of Mycoplasma haemofelis infection and electron microscopic imaging in a cat. The material of present case was included a cat, three years old, male, referred to a history of anorexia, lethargy and dehydration. Clinically were defined dehydration, apathy, tachypnea, 38.2 degrees C body temperature, murmur and tachycardia at the auscultation of heart. Mycoplasma haemofelis was detected at the peripheral smear and using PCR assay, and viewed with scanning electron microscopy. The patient was treated with doxycycline (Tetradox kapsul, Fako (R)) at a dose of 10 mg/kg/day orally for 14 days. The clinical condition of the cat was improved after treatment, which reduced the number of Mycoplasma haemofelis in blood smears, hematological and serum biochemical values were determined to be normal. To the best of authors' knowledge, this case reports the first imaging of scanning electron microscopy of Mycoplasma haemofelis in cats that are naturally infected in Turkey.