Akademik Veri Yönetim Sistemi
Diğer, ss.20-29, 2019
Abstract. The purpose of this research was to investigate the possible protective effect of
melatonin, as a potent antioxidant on I/R-induced renal injury in rats.
Methods. We used 28 female Wistar albino rats weight 200-250g. The rats were randomly
divided into 4 groups. Control Group (C): They were fed with only standard rat diet and
tap water without drug injections or ischemia-reperfusion. Melatonin Group (M): 25 mg/
kg melatonin was administered i.p 30 min. Ischemia/Reperfusion Group (I/R): Rats
were subjected to 45 min of renal pedicle occlusion followed by 24 hours reperfusion.
Melatonin+ischemia/reperfusion Group (M+I/R): Melatonin (25 mg/kg) was
administered 30 min prior to ischemia and immediately before the reperfusion period. Rats
were subjected to 45 min of renal pedicle occlusion followed by 24 hours reperfusion.
Results. While MDA levels increased in the I/R group, SOD and GST activities were seen
to be significantly increased. Although the increase of the SOD activity was observed in
the M+I/R group, no meaningful difference was found. MDA levels were significantly
decreased in M+I/R group compared to the control group, CAT and GST activities were
significantly increased.
Conclusions. Our results show that the treatment with M may prevent kidney damage due
to ischemia result in increasing oxidant stress peroxidation damages further. Melatonin
or its metabolites are capable of neutralizing free radicals and non-radical oxygen-based
reactants. This study suggests that melatonin may be an effective antioxidant agent.
Key words: rat, melatonin, ischemia, reperfusion.