Evaluation of protective effects of melatonin on free radical metabolism in rat kidney during ischemia-reperfusion

Nisari M., Yay A. H. , Ertekin T., Al Ö. , Kaan D. , Nisari M. , ...More

Other, pp.20-29, 2019

  • Publication Type: Other Publication / Other
  • Publication Date: 2019
  • Page Numbers: pp.20-29


Abstract. The purpose of this research was to investigate the possible protective effect of

melatonin, as a potent antioxidant on I/R-induced renal injury in rats.

Methods. We used 28 female Wistar albino rats weight 200-250g. The rats were randomly

divided into 4 groups. Control Group (C): They were fed with only standard rat diet and

tap water without drug injections or ischemia-reperfusion. Melatonin Group (M): 25 mg/

kg melatonin was administered i.p 30 min. Ischemia/Reperfusion Group (I/R): Rats

were subjected to 45 min of renal pedicle occlusion followed by 24 hours reperfusion.

Melatonin+ischemia/reperfusion Group (M+I/R): Melatonin (25 mg/kg) was

administered 30 min prior to ischemia and immediately before the reperfusion period. Rats

were subjected to 45 min of renal pedicle occlusion followed by 24 hours reperfusion.

Results. While MDA levels increased in the I/R group, SOD and GST activities were seen

to be significantly increased. Although the increase of the SOD activity was observed in

the M+I/R group, no meaningful difference was found. MDA levels were significantly

decreased in M+I/R group compared to the control group, CAT and GST activities were

significantly increased.

Conclusions. Our results show that the treatment with M may prevent kidney damage due

to ischemia result in increasing oxidant stress peroxidation damages further. Melatonin

or its metabolites are capable of neutralizing free radicals and non-radical oxygen-based

reactants. This study suggests that melatonin may be an effective antioxidant agent.

Key words: rat, melatonin, ischemia, reperfusion.