In this study, 14 different lichen and moss samples were collected from near the Sivas-Tokat motorway (5-25 m), control samples were collected from uncontaminated locations (1000-3000 m) during 2005. Samples were analyzed using flame and graphite furnace atomic absorption spectrometry after microwave digestion. The maximum metal concentrations were found to be as 468.1 mu g/g (Fe), 270.5 mu g/g (Mn), 67.6 mu g/g (Zn), 53.3 mu g/g (Pb), 79.6 mu g/g (Ni), 33.9 mu g/g (Cr), 29.6 mu g/g (Cu) and 5.7 mu g/g (Cd) for mosses, 455.5 mu g/g (Fe), 170.5 mu g/g (Mn). 77.6 mu g/g (Zn), 6.5 mu g/g (Pb), 10.1 mu g/g (Ni), 3.8 mu g/g (Cr), 25.6 mu g/g(Cu) and 1.5 mu g/g (Cd) mu g/g for lichens.The concentration of trace metals in samples is depended on moss and lichen species. Some species is accumulated trace metals at high ratio. (C) 2008 Elsevier B.V. All rights reserved.