The quick extraction of chitin from an epizoic crustacean species (Chelonibia patula)
NATURAL PRODUCT RESEARCH, cilt.28, sa.23, ss.2186-2190, 2014 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 28 Sayı: 23
- Basım Tarihi: 2014
- Doi Numarası: 10.1080/14786419.2014.927469
- Dergi Adı: NATURAL PRODUCT RESEARCH
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.2186-2190
- Anahtar Kelimeler: barnacle, isolation, chitin, nanofibre, thermal properties
- Erciyes Üniversitesi Adresli: Evet
Özet
Chitin was isolated from the shells of Chelonibia patula (barnacle, Crustacea), which lives on blue crab epizoically, following a 10-min demineralisation process through HCl and a 20-min deproteinisation process through NaOH. Due to the low-crystalline structure, and mineral-rich and low-protein content of the shells, chitin isolation was convenient. It was observed that the shell structure of C. patula contains 3.11% chitin per its dry weight. Following characterisation of the isolated chitin by using Fourier transform infrared spectroscopy, thermogravimetric analysis, X-ray diffractometry, elemental analysis and scanning electron microscopy, it was determined that there was close similarity with the a-chitin isolated from crabs, shrimps and insects in various studies. It was observed that chitin was composed of nanofibres with a width of 10-20 nm. It was concluded that this was an economically advantageous chitin resource compared with crustaceans such as shrimp, crayfish and crab, because it is possible to isolate chitin in a significantly shorter time.