The quick extraction of chitin from an epizoic crustacean species (Chelonibia patula)


Kaya M., Karaarslan M., Baran T., Can E., Ekemen G., Bitim B., ...More

NATURAL PRODUCT RESEARCH, vol.28, no.23, pp.2186-2190, 2014 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 28 Issue: 23
  • Publication Date: 2014
  • Doi Number: 10.1080/14786419.2014.927469
  • Title of Journal : NATURAL PRODUCT RESEARCH
  • Page Numbers: pp.2186-2190

Abstract

Chitin was isolated from the shells of Chelonibia patula (barnacle, Crustacea), which lives on blue crab epizoically, following a 10-min demineralisation process through HCl and a 20-min deproteinisation process through NaOH. Due to the low-crystalline structure, and mineral-rich and low-protein content of the shells, chitin isolation was convenient. It was observed that the shell structure of C. patula contains 3.11% chitin per its dry weight. Following characterisation of the isolated chitin by using Fourier transform infrared spectroscopy, thermogravimetric analysis, X-ray diffractometry, elemental analysis and scanning electron microscopy, it was determined that there was close similarity with the a-chitin isolated from crabs, shrimps and insects in various studies. It was observed that chitin was composed of nanofibres with a width of 10-20 nm. It was concluded that this was an economically advantageous chitin resource compared with crustaceans such as shrimp, crayfish and crab, because it is possible to isolate chitin in a significantly shorter time.