Evaluation of Cytotoxicity of<i> Centaurea</i><i> lycaonica</i> Boiss. & Heldr. Plant on Human Cervical Cancer Cell Line with MTT Test and xCELLigence System

Arslan A. K., Güngörenler E., Paşayeva L., Bozkurt N. M., Tugay O.

KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE, cilt.28, sa.1, ss.47-52, 2025 (ESCI, TRDizin) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 1
  • Basım Tarihi: 2025
  • Doi Numarası: 10.18016/ksutarimdoga.vi.1496499
  • Dergi Adı: KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Agricultural & Environmental Science Database, CAB Abstracts, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.47-52
  • Anahtar Kelimeler: Centaurea lycaonica, Cervical cancer, HeLa, MTT, xCELLigence
  • Erciyes Üniversitesi Adresli: Evet

Özet

Many species belonging to the genus Centaurea L. are endemic in various parts of the world and are used in folk medicine. The cytotoxicity of some species belonging to this genus on cervical cancer cell lines was investigated. In this study, it was aimed to investigate the cytotoxic effect of dichloromethane (CRD) and methanol (CRM) extracts prepared by 24 h maceration method on human cervical cancer cell line (HeLa) at 48 h exposure based on the root part of Centaurea lycaonica, which is endemic to Turkey and about which there are almost no studies. The effect of different concentrations of plant extracts on cell viability was investigated using MTT and xCELLigence RTCA systems, which have different working principles, and IC50 values were determined. As a result, MTT findings of CRD and CRM extracts were consistent with xCELLigence analysis and found to have a cytotoxic effect on HeLa cells. It was thought that C. lycaonica species could be evaluated as a new natural source in cancer treatment. It is thought that this study will encourage the elucidation of the biological activities and mechanisms of action of many species endemic to Turkey.